Construction and expression of the fusion gene of H pylori HspA subunit and E.coli heat-labile enterotoxin B subunit
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چکیده
منابع مشابه
Construction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
متن کاملESCHERICHIA COLI HEAT-LABILE TOXIN B SUBUNIT: CONSTRUCTION AND EVALUATION OF PLASMIDS PROVIDING CONTROLLED HIGH LEVEL PRODUCTION OF THE PROTEIN
With the plasmid DNA from a clinical isolate of enterotoxigenic Escherichia coli (ETEC) H 10407 as template, PCR-mediated cloning of the sequence encoding the heat-labile toxin B subunit (L T -B) has been carried out. Then this sequence was recloned into the pTrc 99A and pET23a expression vectors to give the pJasmids pTRCLTB and pETLTB, respectively. After induction, the former plasmid provides...
متن کاملconstruction and expression of a fused gene for b subunit of the heat-labile and a truncated form of the heat-stable enterotoxins in escherichia coli
elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
متن کاملPreparation of biocompatible heat-labile enterotoxin subunit B-bovine serum albumin nanoparticles for improving tumor-targeted drug delivery via heat-labile enterotoxin subunit B mediation
Heat-labile enterotoxin subunit B (LTB) is a non-catalytic protein from a pentameric subunit of Escherichia coli. Based on its function of binding specifically to ganglioside GM1 on the surface of cells, a novel nanoparticle (NP) composed of a mixture of bovine serum albumin (BSA) and LTB was designed for targeted delivery of 5-fluorouracil to tumor cells. BSA-LTB NPs were characterized by dete...
متن کاملExpression of functional pentameric heat-labile enterotoxin B subunit of Escherichia coli in Saccharomyces cerevisiae.
Although the Escherichia coli heat-labile enterotoxin B subunit (LTB) has already been expressed in several different systems, including prokaryotic and eukaryotic organisms, studies regarding the synthesis of LTB into oligomeric structures of pentameric size in the budding yeast Saccharomyces cerevisiae have been limited. Therefore, this study used a functional signal peptide of the amylase 1A...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2003
ISSN: 2219-2859
DOI: 10.11569/wcjd.v11.i10.1475